Isolation and localization of cells expressing Sca-1 in the Adult Mouse Ovary: An evidence for presence of Mesenchymal Stem cells
Keywords:
Ovary, Stem cell, Sca-1, Surface epitheliumAbstract
Objective: Recently growing evident declared that ‘neo-oogenesis’ continues in mature female life span and simultaneously another studies confirmed the presence of spermatogonial stem cells (SSCs). Even though there is agreement between scientist about SSCs population in male gender but on the other side ovarian stem cells have received raising challenges regarding the existence in the surface epithelium of ovary. Mesenchymal stem cells (MSCs) are the most applicable source of stem cells and the common marker of MSCs is Sca-1 so the purpose of this study was to clarify the incidence of stem cells in the surface epithelium of ovary
Methods: forty C57BL6 mice were sacrificed and the ovary carefully excised from its surrounding fat tissue, after mechanical and enzymatic digestion cells were stained with Sca1 to estimate the incidence of positive stem cells (SCs) population fluorescence activated cell sorting (FACS). Part of digested cells used for RT-PCR, also histological section prepared for immunohistochemical (IHC) staining of Sca-1 in ovarian surface epithelium (OSE) FACS.
Results: The gene expression of Sca-1 was confirmed in the ovarian tissue. As well, localization of Sca-1 positive cells was detected in the germinal layer of ovary and epithelial granular layer of primordial follicles. Moreover, we successfully could isolated the Sca-1positive cells through
Conclusion: The present work findings confirmed an inclusive stem cell population in the ovary which can be a strong evident for regeneration of ovarian tissue in either purpose of ovulation scar and neo-oogenesis.
References
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2. Hanna CB, Hennebold JD. Ovarian germline stem cells: an unlimited source of oocytes? Fertility and sterility. 2014;101(1):20-30.
3. Liu Y, Wu C, Lyu Q, Yang D, Albertini DF, Keefe DL, et al. Germline stem cells and neo-oogenesis in the adult human ovary. Developmental biology. 2007;306(1):112-20.
4. Woods DC, Tilly JL. Isolation, characterization and propagation of mitotically active germ cells from adult mouse and human ovaries. Nature protocols. 2013;8(5):966-88.
5. Johnson J, Bagley J, Skaznik-Wikiel M, Lee H-J, Adams GB, Niikura Y, et al. Oocyte generation in adult mammalian ovaries by putative germ cells in bone marrow and peripheral blood. Cell. 2005;122(2):303-15.
6. Eliazer S, Buszczak M. Finding a niche: studies from the Drosophila ovary. Stem cell research & therapy. 2011;2(6):45.
7. Parte S, Bhartiya D, Patel H, Daithankar V, Chauhan A, Zaveri K, et al. Dynamics associated with spontaneous differentiation of ovarian stem cells in vitro. Journal of ovarian research. 2014;7(1):25.
8. Bui H-T, Van Thuan N, Kwon D-N, Choi Y-J, Kang M-H, Han J-W, et al. Identification and characterization of putative stem cells in the adult pig ovary. Development. 2014;141(11):2235-44.
9. Virant-Klun I, Skutella T. Stem cells in aged mammalian ovaries. Aging (Albany NY). 2010;2(1):3.
10. Dzafic E, Stimpfel M, Novakovic S, Cerkovnik P, Virant-Klun I. Expression of mesenchymal stem cells-related genes and plasticity of aspirated follicular cells obtained from infertile women. BioMed research international. 2014;2014.
11. Houlihan DD, Mabuchi Y, Morikawa S, Niibe K, Araki D, Suzuki S, et al. Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α. Nature protocols. 2012;7(12):2103-11.
12. Yazdekhasti H, Hosseini MA, Rajabi Z, Parvari S, Salehnia M, Koruji M, et al. Improved Isolation, Proliferation, and Differentiation Capacity of Mouse Ovarian Putative Stem Cells. Cellular Reprogramming (Formerly" Cloning and Stem Cells"). 2017;19(2):132-44.
13. Virantâ€Klun I, Zech N, Rožman P, Vogler A, CvjetiÄanin B, Klemenc P, et al. Putative stem cells with an embryonic character isolated from the ovarian surface epithelium of women with no naturally present follicles and oocytes. Differentiation. 2008;76(8):843-56.
14. Park E-S, Woods DC, Tilly JL. Bone morphogenetic protein 4 promotes mammalian oogonial stem cell differentiation via Smad1/5/8 signaling. Fertility and sterility. 2013;100(5):1468-75. e2.
15. Bhartiya D, Sriraman K, Parte S. Stem cell interaction with somatic niche may hold the key to fertility restoration in cancer patients. Obstetrics and gynecology international. 2012;2012.
16. Tilly JL, Rueda BR. Stem cell contribution to ovarian development, function, and disease. Endocrinology. 2008;149(9):4307-11.
17. da Silva Meirelles L, Chagastelles PC, Nardi NB. Mesenchymal stem cells reside in virtually all post-natal organs and tissues. Journal of cell science. 2006;119(11):2204-13.
18. Morikawa S, Mabuchi Y, Kubota Y, Nagai Y, Niibe K, Hiratsu E, et al. Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow. Journal of Experimental Medicine. 2009;206(11):2483-96.
19. Dicker A, Le Blanc K, Åström G, van Harmelen V, Götherström C, Blomqvist L, et al. Functional studies of mesenchymal stem cells derived from adult human adipose tissue. Experimental cell research. 2005;308(2):283-90.
20. Stappenbeck TS, Miyoshi H. The role of stromal stem cells in tissue regeneration and wound repair. Science. 2009;324(5935):1666-9.
21. Houlihan DD, Mabuchi Y, Morikawa S, Niibe K, Araki D, Suzuki S, et al. Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α. Nature Protocols. 2012;7:2103.
22. Morrison SJ, Spradling AC. Stem cells and niches: mechanisms that promote stem cell maintenance throughout life. Cell. 2008;132(4):598-611.
23. Khosravi-Farsani S, Amidi F, Roudkenar MH, Sobhani A. Isolation and enrichment of mouse female germ line stem cells. Cell Journal (Yakhteh). 2015;16(4):406.
24. Hamidabadi HG, Sobhani A, Bojnordi MN. Multipotent SSEA-1 Positive Cells Population Differentiation into Primordial Germ Cells and Subsequently Progress into Oocyte-like Cells. Archives of Iranian Medicine (AIM). 2015;18(7).
25. Zou K, Yuan Z, Yang Z, Luo H, Sun K, Zhou L, et al. Production of offspring from a germline stem cell line derived from neonatal ovaries. Nature cell biology. 2009;11(5):631-6.
26. Lu Z, Wu M, Zhang J, Xiong J, Cheng J, Shen W, et al. Improvement in isolation and identification of mouse oogonial stem cells. Stem cells international. 2016;2016.
27. Yazdekhasti H, Rajabi Z, Parvari S, Abbasi M. Used protocols for isolation and propagation of ovarian stem cells, different cells with different traits. Journal of ovarian research. 2016;9(1):68.
28. Parvari S, Yazdekhasti H, Rajabi Z, Gerayeli Malek V, Rastegar T, Abbasi M. Differentiation of mouse ovarian stem cells toward oocyte-like structure by coculture with granulosa cells. Cellular Reprogramming (Formerly" Cloning and Stem Cells"). 2016;18(6):419-28.
29. Gamwell LF, Collins O, Vanderhyden BC. The Mouse Ovarian Surface Epithelium Contains a Population of LY6A (SCA-1) Expressing Progenitor Cells That Are Regulated by Ovulation-Associated Factors 1. Biology of reproduction. 2012;87(4):Article 80, 1-10.
30. Johnson J, Canning J, Kaneko T, Pru JK, Tilly JL. Germline stem cells and follicular renewal in the postnatal mammalian ovary. Nature. 2004;428(6979):145-50.
31. Bukovsky A, Svetlikova M, Caudle MR. Oogenesis in cultures derived from adult human ovaries. Reproductive Biology and Endocrinology. 2005;3(1):1.
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Published
2018-06-26
How to Cite
Majidi Zolbin, M., Mokhtari, T., Amidi, F., Parvari, S., MirgaloyBayat, S., & Abbasi, M. (2018). Isolation and localization of cells expressing Sca-1 in the Adult Mouse Ovary: An evidence for presence of Mesenchymal Stem cells. Journal of Contemporary Medical Sciences, 4(2), 70–73. Retrieved from https://www.jocms.org/index.php/jcms/article/view/401
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