Triiodothyronine Improves Morphology and Up-regulates seladin-1 of neurospheres extracted from Subventricular Zone in Streptozotocin-induced rat model of Alzheimer's disease

  • Tahmineh Mokhtari CAS Key Laboratory of Mental Health, Institute of Psychology, Beijing, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing, China.
  • Simin Mahahakizadeh Department of Anatomy, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
  • Hadi Aligholi Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Sahar Ijaz Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Anatomy & Histology, Faculty of Biosciences, University of Veterinary & Animal Sciences, Lahore, Pakistan.
  • Leila Noori Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Gholamreza Hassanzadeh Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Neuroscience and addiction studies, School of advanced technologies in medicine, Tehran University of Medical Sciences, Tehran, Iran; Legal medicine research center, Legal Medicine Organization, Tehran, Iran.


Objectives: In this study, the effects of triiodothyronine (T3) on neurospheres isolated from SVZ of AD induced rats were examined.
Methods: Eighteen male Wistar rats were classified into two groups: Sham (Sh) and STZ (Streptozotocin injected, 1.5 mg/kg in each lateral ventricle on days 1 and 3 after recovery). On day 21, the SVZ was extracted and neurospheres were cultured. T3 (50 nM) was added to the culture medium (STZ+T3 group) and then, the morphology and seladin-1 gene expression of neurospheres were evaluated.  
Results: The diameter and the number of neurospheres along with the gene expression of seladin-1 were significantly decreased in the STZ group compared to Sh group (P˂0.05) while the administration of T3 significantly (P˂0.05) increased all these parameters in the STZ group.
Conclusion: STZ decreases the proliferation of stem cells extracted from SVZ and administration of T3 to the culture media improves the morphology and up-regulates the gene expression of seladin-1 of neurospheres.
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How to Cite
MOKHTARI, Tahmineh et al. Triiodothyronine Improves Morphology and Up-regulates seladin-1 of neurospheres extracted from Subventricular Zone in Streptozotocin-induced rat model of Alzheimer's disease. Journal of Contemporary Medical Sciences, [S.l.], v. 6, n. 1, apr. 2020. ISSN 2413-0516. Available at: <>. Date accessed: 13 july 2020.