Characteristics of Vitamin D3 Receptor Genotypes in T2DM of Iraqi Obese Women


  • Baraa Abdul-Kareem Mutar Department of Chemistry and Biochemistry, College of Medicine, University of Kerbala, Kerbala, Iraq.
  • Thikra Ali Allwsh Department of Chemistry, College of Science, University of Mosul, Mosul, Iraq.
  • Ammar Gany Yassin Department of Chemistry and Biochemistry, College of Medicine, University of Kerbala, Kerbala, Iraq.
  • Fadhil Jawad Al-Tu’ma Department of Chemistry and Biochemistry, College of Medicine, University of Kerbala, Kerbala, Iraq.
  • Hassanien S. Taghi Department of Pharmaceutics, College of Pharmacy, Al-Bayan University, Iraq.



Diabetes Mellitus, Type 2, Receptors, Calcitriol, Vitamin D-Binding Protein, HSP-70


Objective: This study aims to examine the association between the (rs1544410) polymorphism of the VDR gene with the pathogenesis of T2DM in Iraqi obese women.

Methods: A case-control study was performed on 50 patients with T2DM and 50 apparently healthy subjects who were admitted to Al-Hussein Teaching Hospital and Al-Hassan Center of Diabetes and Endocrinology unit / Kerbala health directorate – Iraq during (April 2022 – March 2023). The T2DM groups were divided into two groups, 25 obese and 25 non-obese; the control group was divided into 25 obese and 25 non-obese as apparently healthy groups. The ELISA Kit was used to measure serum 25(OH)D3, heat shock protein-70, VDBP, insulin and C-peptide. Also HbA1c% and insulin resistance (HOMA-IR) were evaluated. The vitamin D3 receptor gene (VDR) variant and the SNP (rs1544410) polymorphism was determined using allele specific polymerase chain reaction, 1.5% agarose gel electrophoresis and then visualized by gel photo-documentation system.

Results: The result of vitamin D3 variants genotype (rs 1544410) was a clear band with a molecular size of 200 bps. The size of the amplicon was determined by compare with DNA ladder 100 - 1500 bp. The result of the comparison between observed and anticipated values for SNIP with (rs 1544410) in the tested population was statistically significant, P= < 0.001 and the difference between demographic characteristics and (rs 1544410) SNP, age and BMI shows non-significant difference among all groups. The difference between biomarkers and (rs1544410) SNP was performed using one-way ANOVA test to compare the mean levels of HSP-70, VDBP, C-peptide,  RBC and HbA1c% which shown a non-significant difference among the variants of VDBP Genotype (rs1544410) in  obese women (patients and control) studied groups, p value > 0.05.  

Conclusion: The logistic analysis of the (rs1544410) SNP of the patients concluded that HSP-70, VDBP, and C-peptide  level was no significantly related to the also C-peptide, was shown to be a related risk factor to both CT and CT alleles (1.003, p > 0.05) in comparison with CC alleles. Furthermore, HbA1c% level was demonstrated to be related as a risk factor for the CG allele in comparison with CC and GG alleles (1.009, p < 0.05).


Zou, Q., Qu, K., Luo, Y., Yin, D., Ju, Y., & Tang, H. (2018). Predicting diabetes mellitus with machine learning techniques. Frontiers in Genetics, 9, 515.

Zheng, Y., Ley, S. H., & Hu, F. B. (2018). Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nature Reviews Endocrinology, 14(2), 88–98.

Padhi, S., Nayak, A. K., & Behera, A. (2020). Type II diabetes mellitus: A review on recent drug based therapeutics. Biomedicine & Pharmacotherapy, 131, 110708.

Pischon, T., Boeing, H., Hoffmann, K., Bergmann, M., Schulze, M. B., Overvad, K., Van Der Schouw, Y., Spencer, E., Moons, K., & Tjønneland, A. (2008). General and abdominal adiposity and risk of death in Europe. New England Journal of Medicine, 359(20), 2105–2120.

Horst-Sikorska, W., Dytfeld, J., Wawrzyniak, A., Marcinkowska, M., Michalak, M., Franek, E., Napiórkowska, L., Drwęska, N., & Słomski, R. (2013). Vitamin D receptor gene polymorphisms, bone mineral density and fractures in postmenopausal women with osteoporosis. Molecular Biology Reports, 40, 383–390.

Li Y, Xi B, Li K, Wang C. Association between vitamin D receptor gene polymorphisms and bone mineral density in Chinese women. Molecular biology reports. 2012 May;39:5709–17.

Mosaad, Y. M., Hammad, E. M., Fawzy, Z., Aal, I. A. A., Youssef, H. M., ElSaid, T. O., Monir, R., & El-Deek, B. S. (2014). Vitamin D receptor gene polymorphism as possible risk factor in rheumatoid arthritis and rheumatoid related osteoporosis. Human Immunology, 75(5), 452–461.

Manchanda, P., & Bid, H. (2012). Vitamin D receptor and type 2 diabetes mellitus: Growing therapeutic opportunities. Indian Journal of Human Genetics, 18(3), 274.

Sung, C.-C., Liao, M.-T., Lu, K.-C., & Wu, C.-C. (2012). Role of vitamin D in insulin resistance. Journal of Biomedicine and Biotechnology, 2012.

Ortlepp, J., Metrikat, J., Albrecht, M., Von Korff, A., Hanrath, P., & Hoffmann, R. (2003). The vitamin D receptor gene variant and physical activity predicts fasting glucose levels in healthy young men. Diabetic Medicine, 20(6), 451–454.

Anuradha, C. V. (2013). Phytochemicals targeting genes relevant for type 2 diabetes. Canadian Journal of Physiology and Pharmacology, 91(6), 397–411.

Rahmadhani, R., Zaharan, N. L., Mohamed, Z., Moy, F. M., & Jalaludin, M. Y. (2017). The associations between VDR BsmI polymorphisms and risk of vitamin D deficiency, obesity and insulin resistance in adolescents residing in a tropical country. PloS One, 12(6), e0178695.

Karonova, T., Grineva, E., Belyaeva, O., Bystrova, A., Jude, E. B., Andreeva, A., Kostareva, A., & Pludowski, P. (2018). Relationship between vitamin D status and vitamin D receptor gene polymorphisms with markers of metabolic syndrome among adults. Frontiers in Endocrinology, 9, 448.

Pike, J. W., Meyer, M. B., Benkusky, N. A., Lee, S. M., John, H. S., Carlson, A., Onal, M., & Shamsuzzaman, S. (2016). Genomic determinants of vitamin D-regulated gene expression. Vitamins & Hormones, 100, 21–44.

Zaki, M., Kamal, S., Basha, W. A., Youness, E., Ezzat, W., El-Bassyouni, H., & Amr, K. (2017). Association of vitamin D receptor gene polymorphism (VDR) with vitamin D deficiency, metabolic and inflammatory markers in Egyptian obese women. Genes & Diseases, 4(3), 176–182.

Gyapay, G., Schmitt, K., Fizames, C., Jones, H., Vega-Czarny, N., Spillett, D., Muselet, D., Prud’Homme, J.-F., Dib, C., & Auffray, C. (1996). A radiation hybrid map of the human genome. Human Molecular Genetics, 5(3), 339–346.

Mackawy, A. M., & Badawi, M. E. (2014). Association of vitamin D and vitamin D receptor gene polymorphisms with chronic inflammation, insulin resistance and metabolic syndrome components in type 2 diabetic Egyptian patients. Meta Gene, 2, 540–556.

Bouillon, R., Carmeliet, G., Verlinden, L., Van Etten, E., Verstuyf, A., Luderer, H. F., Lieben, L., Mathieu, C., & Demay, M. (2008). Vitamin D and human health: Lessons from vitamin D receptor null mice. Endocrine Reviews, 29(6), 726–776.

Wang, H., Chen, W., Li, D., Yin, X., Zhang, X., Olsen, N., & Zheng, S. G. (2017). Vitamin D and chronic diseases. Aging and Disease, 8(3), 346.

Chiu, K. C., Chu, A., Go, V. L. W., & Saad, M. F. (2004). Hypovitaminosis D is associated with insulin resistance and β cell dysfunction. The American Journal of Clinical Nutrition, 79(5), 820–825.

Knekt, P., Laaksonen, M., Mattila, C., Härkänen, T., Marniemi, J., Heliövaara, M., Rissanen, H., Montonen, J., & Reunanen, A. (2008). Serum vitamin D and subsequent occurrence of type 2 diabetes. Epidemiology, 666–671.

Malik, R., Farooq, R., Mehta, P., Ishaq, S., Din, I., Shah, P., & Majid, S. (2018). Association of Vitamin D receptor gene polymorphism in adults with type 2 diabetes in the Kashmir Valley. Canadian Journal of Diabetes, 42(3), 251–256.

Shaat, N., Ignell, C., Katsarou, A., & Berntorp, K. (2017). Glucose homeostasis, beta cell function, and insulin resistance in relation to vitamin D status after gestational diabetes mellitus. Acta obstetricia et gynecologica Scandinavica, 96(7), 821–827.

Ignell, C., Shaat, N., Ekelund, M., & Berntorp, K. (2013). The impact of ethnicity on glucose homeostasis after gestational diabetes mellitus. Acta Diabetologica, 50, 927–934.

Ogunkolade, B.-W., Boucher, B. J., Prahl, J. M., Bustin, S. A., Burrin, J. M., Noonan, K., North, B. V., Mannan, N., McDermott, M. F., & DeLuca, H. F. (2002). Vitamin D receptor (VDR) mRNA and VDR protein levels in relation to vitamin D status, insulin secretory capacity, and VDR genotype in Bangladeshi Asians. Diabetes, 51(7), 2294–2300.

Al-Daghri, N. M., Al-Attas, O. S., Alkharfy, K. M., Khan, N., Mohammed, A. K., Vinodson, B., Ansari, M. G. A., Alenad, A., & Alokail, M. S. (2014). Association of VDR-gene variants with factors related to the metabolic syndrome, type 2 diabetes and vitamin D deficiency. Gene, 542(2), 129–133.

Karuwanarint, P., Phonrat, B., Tungtrongchitr, A., Suriyaprom, K., Chuengsamarn, S., & Tungtronchitr, R. (2018). Genetic variations of vitamin D receptor gene in metabolic syndrome and related diseases in the Thai population. Asia Pacific Journal of Clinical Nutrition, 27(4), 935–944.

Kamei, Y., Kawada, T., Kazuki, R., Ono, T., Kato, S., & Sugimoto, E. (1993). Vitamin D receptor gene expression is up-regulated by 1, 25-dihydroxyvitamin D3 in 3T3-L1 preadipocytes. Biochemical and Biophysical Research Communications, 193(3), 948–955.




How to Cite

Mutar, B. A.-K. ., Allwsh, T. A. ., Yassin, A. G. ., Al-Tu’ma, F. J. ., & Taghi, H. S. (2023). Characteristics of Vitamin D3 Receptor Genotypes in T2DM of Iraqi Obese Women. Journal of Contemporary Medical Sciences, 9(3), 201–205.

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