Relation of serum and follicular level of BMP15 with Oocyte quality, embryo grading and pregnancy rate

Authors

  • Zainab Hassan Hashim Department of Clinical Reproductive Physiology, High Institution for Infertility Diagnosis and Assisted Reproductive Technologies, University of Al-Nahrain, Baghdad, Iraq.
  • Lubna Amer Institution for Infertility Diagnosis and Assisted Reproductive Technologies, University of Al-Nahrain, Baghdad, Iraq.
  • Estabraq A. Al-Wasiti Department of Biochemistry, College of Medicine, University of Al-Nahrain, Baghdad, Iraq.

DOI:

https://doi.org/10.22317/jcms.v8i5.1283

Keywords:

Follicular Level, BMP15, Oocyte, Embryo Grading, Pregnancy Rate

Abstract

Objectives: Use of serum and follicular fluid concentration of oocyte secreted factors BMP15 as biomarkers of oocyte quality, embryo quality and it's relation to pregnancy rate.

Methods: Eighty eight women were included in this study; they were selected from those undergoing intra-cytoplasmic sperm injection.

Results: Positive pregnancy was achieved by 14 women accounting for 19.0 % (total number of women that reach embryo transfer was 72). No significant difference in mean serum BMP15 between pregnant and non- pregnant women, but the level of follicular fluid BMP15 was significantly higher in pregnant women. MI oocyte count was not significantly correlated to serum or follicular fluid BMP15 (p > 0.05). MII oocyte count showed highly significant positive correlation to serum and follicular fluid BMP15 (p < 0.01). Grade1 embryo count showed highly significant positive correlation to serum and follicular fluid BMP15 (p < 0.01), Grade 2 embryo count showed significant positive correlation to serum BMP15 (p = 0.032), but the grade 2 embryo count showed non- significant correlation to follicular BMP15 (p > 0.05). Also grade 3 embryo count showed non- significant correlation to serum and follicular BMP15 (p > 0.05).

Conclusions: The current study revealed that  serum and follicular BMP15 could be used as indicator for oocyte maturity, and serum BMP15 could be used as indicator of good quality embryos.

References

Adhikari, D., Lee, I. W., Yuen, W. S., and Carroll, J. (2022). Oocyte mitochondria—key regulators of oocyte function and potential therapeutic targets for improving fertility. Biology of Reproduction , 106(2) : 366-377.‏

Mustafa, M., Sharifa, A. M., Hadi, J., IIIzam, E., and Aliya, S. (2019). Male and female infertility: causes, and management. IOSR Journal of Dental and Medical Sciences, 18 : 27-32.‏

Aghajanova, L., Hoffman, J., Mok-Lin, E., and Herndon, C. N. (2017). Obstetrics and gynecology residency and fertility needs: national survey results. Reproductive sciences, 24(3) : 428-434.‏

Sanfins, A., Rodrigues, P. and Albertini, D. F. (2018). GDF-9 and BMP-15 direct the follicle symphony. Journal of assisted reproduction and genetics, 35(10) : 1741-1750.

Da Broi, M. G., Giorgi, V. S. I., Wang, F., Keefe, D. L., Albertini, D. and Navarro, P. A. (2018). Influence of follicular fluid and cumulus cells on oocyte quality: clinical implications. Journal of assisted reproduction and genetics, 35(5) : 735-751.

Al-Tu’ma, F.J. ; Farhan, N. H. and Al-Safi, W.G. (2015) Association between fat mass and obesity gene (re9939609) polymorphism with PCOS women in Iraqi population, Human Int. J. Pharm. Pharm. Res., 5(1) : 62-72.

Hassan, MF. (2020) Original research the frequency of elevated prolactin level in polycystic ovary syndrome women and it’s effect on pregnancy rate. Global J. Public Health Med., 2(1) : 109 – 117.

Al-Lami, H.B. ; Al-Tu’ma, F.J. and Al-Safi, W.G. (2020). Association between anti-Mullerian hormone and other biomarkers with ovarian function in polycystic ovarian syndrome of Iraqi women, J. Contem. Med. Sci, 6(4) : 168-175.

Wand, F. ; Dai, W. ; Yang, XH. ; Guo, YH. And Sun, Yp. (2016). Analyses of optimal body mass index for infertile patients with either polycystic or non-polycystic ovary syndrome during assisted reproductive treatment in China. Sci. Rep. , 6(1) : 1-9.

Al-Faris, N.N ; Al-Tu’ma, F. J. and Al-Safi, W.G. (2017). Assessment of CRP and its correlation with total antioxidant capacity in women with polycystic ovarian syndrome. Iraqi Nat. J. of Chem., 17(1) : 44-57.

Victoria, M., Labrosse, J., Krief, F., Cédrin-Durnerin, I., Comtet, M. and Grynberg, M. (2019). Anti Müllerian hormone: more than a biomarker of female reproductive functions. Journal of gynecology obstetrics and human reproduction, 48(1) : 19-24.

Dayanir, D., Ruso, H., Kalem, Z., Gurgan, T. and Ozogul, C. (2019). Rewarding Conversation Between Oocyte and Cumulus Cells Directs the Process of Folliculogenesis. Gazi Medical Journal, 30(4).‏

De Geyter, C., Calhaz-Jorge, C., Kupka, M. S., Wyns, C., Mocanu, E., Motrenko, T. and Goossens, V. (2018). ART in Europe, 2014: results generated from European registries by ESHRE: The European IVF-monitoring Consortium (EIM) for the European Society of Human Reproduction and Embryology (ESHRE). Human reproduction, 33(9) : 1586-1601.‏

Jassim, W. H., Al-Obaidi, M. T. and Ghazi, H. F. (2021). The Effect of Intrauterine Infusion of Peripheral Blood Mononuclear Cells Culture on Subendometrial Blood Flow in Patients Undergoing ICSI Cycles. Iraqi Journal of Embryos and Infertility Researches, 10(2) : 53-72

Maya, W. D. C., Du Plessis, S. S. and Velilla, P. A. (2020). SARS-CoV-2 and the testis: similarity with other viruses and routes of infection. Reproductive biomedicine online, 40(6) : 763-76

Duarte‐Neto, A. N., Teixeira, T. A., Caldini, E. G., Kanamura, C. T., Gomes‐Gouvêa, M. S., Dos Santos, A. B., and Hallak, J. (2022). Testicular pathology in fatal COVID‐19: A descriptive autopsy study. Andrology, 10(1) : 13-23

Li, J., Li, C., Liu, X., Yang, J., Zhang, Q., Han, W., and Huang, G. (2022). GDF9 concentration in embryo culture medium is linked to human embryo quality and viability. Journal of Assisted Reproduction and Genetics, 39(1) : 117-125.‏

Chandra, V. and Sharma, G. T. (2020). In vitro strategies to enhance oocyte developmental competence. Frontiers in Bioscience-Scholar, 12(1) : 116-136.‏

Romaguera , R. ; Morató, R. ; Jiménez-Macedo, A.R. ; Catalá, M Roura, M ; Paramio, M.T. ; Palomo, M.J. ; Mogas, T. and Izquierdo, D. (2010). Oocyte secreted factors improve embryo developmental competence of COCs from small follicles in prepubertal goats Theriogenology, Oct 1 ; 74(6) : 1050-9.

Michael, J. D., Campanile, G., and Baruselli, P. S. (2020). Transforming growth factor-β superfamily and interferon-τ in ovarian function and embryo development in female cattle: review of biology and application. Reproduction, Fertility and Development, 32(6) : 539-552.‏

Revelli, A., Gennarelli, G., Sestero, M., Canosa, S., Carosso, A., Salvagno, F., and Benedetto, C. (2020). A prospective randomized trial comparing corifollitropin-α late-start (day 4) versus standard administration (day 2) in expected poor, normal, and high responders undergoing controlled ovarian stimulation for IVF. Journal of Assisted Reproduction and Genetics, 37(5) : 1163-1170.‏

Daneshjou, D., Mehranjani, M. S., Zadehmodarres, S., Shariatzadeh , S. M. A. and Mofarahe, Z. S. (2022). Sitagliptin/metformin improves the fertilization rate and embryo quality in polycystic ovary syndrome patients through increasing the expression of GDF9 and BMP15: A new alternative to metformin (a randomized trial). Journal of Reproductive Immunology, 150 : 103499.‏

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Published

2022-10-26

How to Cite

Hashim, Z. H. ., Amer, L. ., & Al-Wasiti, E. A. . (2022). Relation of serum and follicular level of BMP15 with Oocyte quality, embryo grading and pregnancy rate. Journal of Contemporary Medical Sciences, 8(5), 337–342. https://doi.org/10.22317/jcms.v8i5.1283