Comparison of Folliculogenesis and Follicular Maturation in Norvegicus Rats using Foeniculum Vulgare and Clomiphene Citrate

Abstract

Objective: The objective of this work is to understand the importance of follicular maturation in the reproductive life of the Wistar's rats (Rattus norvegicus) using Foeniculum Vulgare (Fennel) and Clomiphene Citrate as reference drug.

Methods: Foeniculum Vulgare (Fennel) are used in doses of (400, 800mg/Kg), Clomiphene Citrate along with distilled water administration. The sexual hormones involved in the oestrous cycle, folliculogenesis, the role of the ovaries, the embryonic and fetal development of the ovary are addressed. An extract of Fennel was obtained using ethyl alcohol by miscreation. After successful administration, a histological study was also conducted on 40 rats grouped into four categories. Statistical analysis also applied standard parameters like chi-square, Tukey test and variance test.

Results: It was identified that after administration of Fennel citrate drug along with distilled waters, significant differences (p<0.05) were identified in the rats and all rats formed primary (90% given 800mg/kg after fennel administration), secondary and matured (80% given 800mg/kg after fennel administration) follicles.

Conclusion: The effect of F. Vulgare at dose of 400mg was not influential as compared to F. Vulgare at 800mg. The clomiphene citrate increased the number of primary follicles in all of the rats studied. In contrast, the rats that ate F. Vulgare, had secondary and mature follicles predominating and did not have any primary follicles.