Interleukin-8 251- A/T polymorphism related to peptic ulcer disease in H. pylori infected patient
Keywords:Helicobacter pylori, molecular biology (PCR), allele-specific oligonucleotide
AbstractObjectives This study includes the investigation of antifungal activity of the local propolis against dermatophytes and yeast.
Methods A total of 92 tissue samples were taken from patients infected with H. pylori and 102 from uninfected individuals has been included. Genetic method used for the detection of H. pylori infection by polymerase chain reaction (PCR) for glM gene identification. IL-8- 251 A/T polymorphism was detected by allele-specific oligonucleotide polymerase chain reaction (ASO- PCR).
Results Among the studied samples, the results improve that genetic polymorphism of IL-8-251 A/T genotype was found in a higher frequency with confidence interval 95% in duodenal ulcer H. pylori infected patient than control.
Conclusion IL-8-251 A allele notice that it has been associated with severe inflammation lead to increase severity of disease.