Fibrin-collagen hydrogel as a scaffold for dermoepidermal skin substitute, preparation and characterization

  • Mahsa Mollapour Sisakht Applied Cell Sciences Department, Kashan University of Medical Science, Kashan, Iran.
  • Mohammad Ali Nilforoushzadeh Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran.
  • Javad Verdi Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Hamid Reza Banafshe Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran
  • Zahra Safaei Naraghi Departments of Dermatology and Pathology, Tehran University of Medical Sciences, Razi Hospital, Tehran, Iran
  • Seyed Abdolreza Mortazavi-Tabatabaei Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran


Objective: Bioengineered skin substitutes were created to address wound healing problems. Skin substitutes contains live human cells seeded onto a matrix to provide cytokine, growth factor and other proteins from ECM required to decrease healing time. These products are classified based on their durability, the cells seeded on them and their originality. In this study, we aimed to investigate fibrin-collagen hydrogel as a new scaffold to design a bilayer temporary skin equivalent.
Methods: Fibrin gel was prepared by crosslinking fibrinogen with thrombin and mixing it with collagen type 1. Human fibroblasts and keratinocytes were isolated from skin biopsies of healthy donors and foreskin and the cells were seeded onto 3D hydrogel layer by layer. Morphological assessment, histological analysis, immunocytochemistry were performed to characterize the scaffold properties.
Result: The results of scaffold characterization demonstrated good porosity, cell viability and biocompatibility of the scaffold.
Conclusion: Fibrin-collagen as a natural material in organotypic cell culture modeling demonstrates that hydrogel scaffolds can be properly designed to generate bilayer or composite temporary skin grafts.
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How to Cite
MOLLAPOUR SISAKHT, Mahsa et al. Fibrin-collagen hydrogel as a scaffold for dermoepidermal skin substitute, preparation and characterization. Journal of Contemporary Medical Sciences, [S.l.], v. 5, n. 1, feb. 2019. ISSN 2413-0516. Available at: <>. Date accessed: 23 mar. 2019.