TY - JOUR AU - Mokhtari, Tahmineh AU - Mahahakizadeh, Simin AU - Aligholi, Hadi AU - Ijaz, Sahar AU - Noori, Leila AU - Hassanzadeh, Gholamreza PY - 2020/02/26 Y2 - 2024/03/28 TI - Triiodothyronine Improves Morphology and Up-regulates seladin-1 of neurospheres extracted from Subventricular Zone in Streptozotocin-induced rat model of Alzheimer's disease JF - Journal of Contemporary Medical Sciences JA - J. Contemp. Med. Sci. VL - 6 IS - 1 SE - Articles DO - 10.22317/jcms.v6i1.735 UR - https://www.jocms.org/index.php/jcms/article/view/735 SP - 32-38 AB - <p><strong>Objectives: </strong>In this study, the effects of triiodothyronine (T3) on neurospheres isolated from SVZ of AD induced rats were examined.</p><p><strong>Methods:</strong> Eighteen male Wistar rats were classified into two groups: Sham (Sh) and STZ (Streptozotocin injected, 1.5 mg/kg in each lateral ventricle on days 1 and 3 after recovery). On day 21, the SVZ was extracted and neurospheres were cultured. T3 (50 nM) was added to the culture medium (STZ+T3 group) and then, the morphology and seladin-1 gene expression of neurospheres were evaluated. &nbsp;</p><p><strong>Results</strong>: The diameter and the number of neurospheres along with the gene expression of seladin-1 were significantly decreased in the STZ group compared to Sh group (PË‚0.05) while the administration of T3 significantly (PË‚0.05) increased all these parameters in the STZ group.</p><p><strong>Conclusion: </strong>STZ decreases the proliferation of stem cells extracted from SVZ and administration of T3 to the culture media improves the morphology and up-regulates the gene expression of seladin-1 of neurospheres.</p> ER -