TY - JOUR AU - Naif, Hassan M PY - 2017/12/26 Y2 - 2024/03/28 TI - Association of gene polymorphism and serum levels of IL-12p40 with the susceptibility to non-Hodgkin's lymphoma in Iraq JF - Journal of Contemporary Medical Sciences JA - J. Contemp. Med. Sci. VL - 3 IS - 12 SE - Articles DO - UR - https://www.jocms.org/index.php/jcms/article/view/268 SP - AB - <p><strong><span style="left: 102.759px; top: 344.692px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.779519);">Objective: </span></strong><span style="left: 162.086px; top: 345.135px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.829504);">Non-Hodgkin lymphomas are a diverse lymphoid neoplasms and it is the sixth most common cancer in the world, but it is well </span><span style="left: 102.759px; top: 364.309px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.80475);">established that immune dysregulation especially by cytokines is one of major risk factors. This study investigates the association of genetic </span><span style="left: 102.759px; top: 383.483px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.819076);">polymorphisms of IL-12p401188A/C and its circulating serum measurement in patients with advanced stage of NHLs in Iraq. </span></p><p><strong><span style="left: 102.759px; top: 402.198px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.789905);">Methods: </span></strong><span style="left: 158.603px; top: 402.642px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.827306);">Fifty-five confirmed patients with advanced stages of NHLs and 40 apparently healthy individuals were used. Genetic </span><span style="left: 102.759px; top: 421.816px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.788076);">polymorphism and circulating levels of IL-12p40 were examined by allele-specific polymerase chain reaction (AS-PCR) using specific </span><span style="left: 102.759px; top: 440.974px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.786861);">primers and ELISA, respectively. </span></p><p><strong><span style="left: 102.759px; top: 459.705px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.756998);">Results </span></strong><span style="left: 150.607px; top: 460.148px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.750634);">The results of SNP IL-12p40 1188A&gt;C had three genotypes, which were AA, AC and CC. These genotypes represented 32.73%, </span><span style="left: 102.759px; top: 479.307px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.793394);">38.18% and 29.09%, respectively, among NHL patients and 52.5%, 37.5% and 10%, respectively, among controls with a significant difference </span><span style="left: 102.759px; top: 498.481px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.796967);">for the homozygous mutant genotype (OR = 4.667, 95% CI = 1.319–16.512, </span><span style="left: 530.512px; top: 498.576px; font-size: 15.8333px; font-family: sans-serif;">P</span><span style="left: 537.463px; top: 498.481px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.786891);"> = 0.017). Serum levels of IL-12p40 were significantly increased </span><span style="left: 102.759px; top: 517.639px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.82478);">in an allele-dependent manner, which was linked to the CC homozygosity as being the highest. </span></p><p><strong><span style="left: 102.759px; top: 536.37px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.742104);">Conclusions: </span></strong><span style="left: 174.626px; top: 536.813px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.841312);">This study has revealed a significant correlation between the gene polymorphisms of IL-12p40 and the induction of serum </span><span style="left: 102.759px; top: 555.972px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.8347);">IL-12 with the risk to poor prognosis in patients with NHL. The correlation with the NHL grades and the prognostic value warrant further </span><span style="left: 102.759px; top: 575.146px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.812514);">progressive investigation of developing prognostic biomarkers for NHL using patients at various disease stages.</span></p> ER -