@article{Mahakizadeh_Akbari_Sharifzadeh_Rastegar_Abolhassani_Hassanzadeh_2018, title={Wharton’ jelly mesenchymal stem cells and insulin effect on BDNF expression in CA1 and CA3 regions of rats’ hippocampus after chronic hypoxia}, volume={4}, url={https://www.jocms.org/index.php/jcms/article/view/400}, abstractNote={<p>Objectives Brain is vulnerable to deprivation of oxygen supply during hypoxia, and therefore undergoes neurodegeneration and cognitive<br>dysfunction. Regarded to Regenerative capacities of Wharton’s jelly mesenchymal stem cells (MSCs) and insulin at the site of injury, we<br>were aimed to evaluate the effect of Wharton’s jelly MSCs and insulin on degenerative consequences induced by chronic hypoxia.<br>Methods 36 male rats were randomly divided into six groups: Control (C), Sham1-saline (Sh1), Sham2-surgery (Sh2), Hypoxia (H), Hypoxia<br>+ Insulin (HI), Hypoxia + MSCs (HCs). Animals were exposed to hypoxic chamber (8% O2, 92% N2) for 30 days (4 hours/day) in H, HI and<br>HCs groups. Intranasal insulin and stereotaxical MSCs in HI and HCs were used, respectively. Spatial learning and memory were analyzed<br>using the Morris water maze task. Brain-derived neurotrophic factor (BDNF) gene expression was studied in the hippocampus by real<br>time-PCR.<br>Results BDNF had the significant depletion in HI group and magnification in HI and HCs groups comparing with C and Sh groups (P < 0.05).<br>Insulin and MSCs improve hypoxia’s signs such as BDNF gene expression fallen and memory impairment.<br>Conclusion In conclusion, we indicated that use of insulin hormone and MSCs as neuroprotective and stimulating factors for neurogenesis,<br>could be beneficial in neurodegenerative damage induced by hypoxia.<br><br></p>}, number={2}, journal={Journal of Contemporary Medical Sciences}, author={Mahakizadeh, Simin and Akbari, Mohammad and Sharifzadeh, Mohammad and Rastegar, Tayebeh and Abolhassani, Farid and Hassanzadeh, Gholamreza}, year={2018}, month={Jun.}, pages={63–69} }