@article{Alizamir_Akbari_Mokhtari_khaksarian_Hassanzadeh_2017, title={Associated functional motor recovery induced by Intracerebroventricular (ICV) microinjection of Wharton’s jelly mesenchymal stem cells following brain ischemia/reperfusion injury in rat: Decreased dark neurons and Bax gene expression in the cerebral cortex}, volume={3}, url={https://www.jocms.org/index.php/jcms/article/view/288}, abstractNote={<p><strong><span style="left: 102.759px; top: 495.568px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.723595);">Objectives: </span></strong><span style="left: 160.091px; top: 494.744px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.82828);">Stroke is a situation caused activation of some events leading to neuronal damage and death. The proteins of Bcl-2-family are </span><span style="left: 102.759px; top: 513.918px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.823594);">important in regulation of cell death and life. Bax, as a Bcl-2-interacting protein, is a member of this family which promotes apoptosis and </span><span style="left: 102.759px; top: 533.077px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.83576);">cell death. Some therapeutic approaches have been introduced for the treatment of ischemic brain injury. Neuroprotection is one of the </span><span style="left: 102.759px; top: 552.251px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.780288);">approaches for diminishing neurological deficits. We investigated the effects of intracerebroventricular (ICV) injection of Wharton’s jelly </span><span style="left: 102.759px; top: 571.425px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.808534);">mesenchymal stem cells (WJ-MSCs) on the cortex of the brain in ischemic rats.</span></p> <p><strong><span style="left: 102.759px; top: 591.407px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.750762);">Methods: </span></strong><span style="left: 151.339px; top: 590.583px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.812343);">In this study, we occlude the middle cerebral artery (MCA) for induction of ischemic stroke in the brain. Rats were classified in four </span><span style="left: 102.759px; top: 609.758px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.759912);">groups of Co, Sh, MCAo and MCAo + WJ-MSCs. Single dose of intraventricular microinjection of WJ-MSCs was injected by a Hamilton </span><span style="left: 102.759px; top: 628.916px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.804718);">syringe. For detecting behavioral outcomes in the rats, Neurological examination was carried out. After 21 days, the animals were sacrificed </span><span style="left: 102.759px; top: 648.09px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.817495);">and their brain tissues were removed for histopathological and molecular analysis.</span></p> <p><strong><span style="left: 102.759px; top: 668.072px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.713476);">Results: </span></strong><span style="left: 143.019px; top: 667.248px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.809211);">ICV microinjection of WJ-MSCs significantly prevented apoptosis and cell death compared with MCAo group. A significant reduction </span><span style="left: 102.759px; top: 686.423px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.816901);">in the level of Bax gene expression was observed in the MCAo + WJ-MSCs as group compared with Co, Sh and MCAo groups (</span><span style="left: 834.512px; top: 686.518px; font-size: 15.8333px; font-family: monospace;">P</span><span style="left: 841.463px; top: 686.423px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.816514);"> < 0.05). </span><span style="left: 102.759px; top: 705.581px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.807408);">H&E staining showed considerable reduction of dark neurons in MCAo + WJ-MSCs group rather than Co, Sh and MCAo groups (</span><span style="left: 831.709px; top: 705.676px; font-size: 15.8333px; font-family: monospace;">P</span><span style="left: 838.66px; top: 705.581px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.797821);"> < 0.05).</span></p> <p><strong><span style="left: 102.759px; top: 725.578px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.704439);">Conclusions: </span></strong><span style="left: 166.804px; top: 724.755px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.818899);">The results of the current study suggest that ICV microinjection of WJ-MSCs had neuroprotective effects on the brain cortex of </span><span style="left: 102.759px; top: 743.913px; font-size: 15.8333px; font-family: sans-serif; transform: scaleX(0.815344);">ischemic rats by reduction of the Bax gene expression level and the number of dark neurons.</span></p>}, number={12}, journal={Journal of Contemporary Medical Sciences}, author={Alizamir, Tahereh and Akbari, Mohammad and Mokhtari, Tahmineh and khaksarian, Mojtaba and Hassanzadeh, Gholamreza}, year={2017}, month={Dec.} }