@article{Tahseen Ibrahim_A. Mussa_Jabbar Fahad Al-Mathkhury_2023, title={A potential role of extracellular DNA in biofilm and ciprofloxacin resistance}, volume={9}, url={https://www.jocms.org/index.php/jcms/article/view/1338}, DOI={10.22317/jcms.v9i2.1338}, abstractNote={<p><strong>Objectives</strong>: This study aims to broaden our knowledge of the role of eDNA in bacterial biofilms and antibiotic-resistance gene transfer among isolates.</p> <p><strong>Methods:</strong> <em>Staphylococcus aureus, E. coli, </em>and<em> Pseudomonas aeruginosa</em> were isolated from different non-repeated 170 specimens. The bacterial isolates were identified using morphological and molecular methods. Different concentrations of genomic DNA were tested for their potential role in biofilms formed by study isolates employing microtiter plate assay. Ciprofloxacin resistance was identified by detecting a mutation in<em> gyrA</em> and <em>parC</em>.</p> <p><strong>Results:</strong> The biofilm intensity significantly decreased (P < 0.05) concerning <em>S. aureus </em>isolates and insignificantly (P > 0.05) concerning <em>E. coli</em> isolates<em>. </em>Yet, one <em>E. coli</em> isolate’s biofilm was significantly decreased (P < 0.05) linearly with increasing eDNA. Of considerable interest, the addition of eDNA led to a significant increase (P < 0.05) in the biofilm of the two-tested <em>P. aeruginosa</em> isolates. Moreover, eDNA participated in transferring Ciprofloxacin resistance to the sensitive isolate when it presents in its biofilm.</p> <p><strong>Conclusion:</strong> eDNA has a dual effect on bacterial biofilms either supportive or suppressive following bacterial species <em>per se</em>. Also, it seems to play an important role in antibiotic resistance within the biofilm.</p>}, number={2}, journal={Journal of Contemporary Medical Sciences}, author={Tahseen Ibrahim, Hind and A. Mussa, Ali and Jabbar Fahad Al-Mathkhury, Harith}, year={2023}, month={Apr.} }