Eremostachys Binalodensis, A Potential Therapeutic Choice for Gingival Inflammatory Wounds

  • Armin S.Hariri Oral and Maxillofacial Medicine Department, Faculty of Dentistry, Aja University of Medical Sciences, Tehran, Iran
  • Sevda Shayesteh Department of Pharmacology and Toxicology, Faculty of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran
  • Parina Asgharian Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
  • Mohsen Chamanara Department of Pharmacology, Faculty of Medicine, Aja University of Medical Sciences, Tehran, Iran
  • Maryam-Sadat Sadrzadeh-Afshar Oral and Maxillofacial Medicine Department, Faculty of Dentistry, Aja University of Medical Sciences, Tehran, Iran http://orcid.org/0000-0001-8277-3805

Abstract

Objectives: In this study we aimed to evaluate the effect of E. binalodensis on gingival inflammatory wounds.
Methods: In-vitro wound was induced by scratching the surface layer of human gingival fibroblasts (hGFs). Cells were pretreated with 1,10,100,1000 µg/ml of E. binalodensis methanol extract prior to 1µg/ml LPS stimulation. hGFs proliferation was assessed by MTT test. Also levels of critical inflammatory cytokines such as IL-1𝛽, IL-6 and TNF-𝛼 were determined by enzyme-linked immunosorbent assay (ELISA).
Results: Wound induction was associated with secretion of IL-1β, IL-6 and TNF-α from hGFs. E. binalodensis enhanced the hGFs proliferation besides reducing the level of IL-1β, IL-6 and TNF-α in LPS-scratch-stimulated hGFs.
Conclusions: Regarding anti-inflammatory and proliferative effects of E. binalodensis on hGFs, availability and safety of it, it is suggested for enhancing the wound healing process in gingival inflammatory wounds.
share this Article by

References

1. Smith PC, Cáceres M, Martínez C, Oyarzún A, Martínez J. Gingival Wound Healing:An Essential Response Disturbed by Aging? Journal of Dental Research. 2015;94(3):395-402.
2. Bhattacharya R, Xu F, Dong G, Li S, Tian C, Ponugoti B, et al. Effect of bacteria on the wound healing behavior of oral epithelial cells. PloS one. 2014;9(2):e89475.
3. Palmqvist P, Lundberg P, Lundgren I, Hänström L, Lerner U. IL-1β and TNF-α regulate IL-6-type cytokines in gingival fibroblasts. Journal of dental research. 2008;87(6):558-63.
4. Shirasugi M, Nishioka K, Yamamoto T, Nakaya T, Kanamura N. Normal human gingival fibroblasts undergo cytostasis and apoptosis after long-term exposure to butyric acid. Biochemical and biophysical research communications. 2017;482(4):1122-8.
5. Basso FG, Soares D, Pansani T, Turrioni A, Scheffel D, de Souza Costa C, et al. Effect of LPS treatment on the viability and chemokine synthesis by epithelial cells and gingival fibroblasts. Archives of oral biology. 2015;60(8):1117-21.
6. Taylor JJ. Protein biomarkers of periodontitis in saliva. International Scholarly Research Notices. 2014;2014.
7. Roques C, Téot L. The use of corticosteroids to treat keloids: a review. The international journal of lower extremity wounds. 2008;7(3):137-45.
8. Mozaffarian V. A dictionary of Iranian plant names. Tehran: Farhang Moaser. 1996;396.
9. Asgharian P, Delazar A, Asnaashari S. Chemical Constituents of Eremostachys macrophylla Montbr. & Auch. Aerial Parts. Pharmaceutical Sciences. 2020;26(2):203-8.
10. Mosaddegh M, Naghibi F, Moazzeni H, Pirani A, Esmaeili S. Ethnobotanical survey of herbal remedies traditionally used in Kohghiluyeh va Boyer Ahmad province of Iran. Journal of ethnopharmacology. 2012;141(1):80-95.
11. Khan S, Nisar M, Simjee SU, Rehman W, Khan R, Jan I, et al. Evaluation of micronutrients level and antinociceptive property of Eremostachys laciniata (L) Bunge. African Journal of Biotechnology. 2010;9(5).
12. Delazar A, Sarker SD, Nahar L, Jalali SB, Modaresi M, Hamedeyazdan S, et al. Rhizomes of Eremostachys laciniata: isolation and structure elucidation of chemical constituents and a clinical trial on inflammatory diseases. Advanced pharmaceutical bulletin. 2013;3(2):385.
13. Khan S, Nisar M, Rehman W, Khan R, Nasir F. Anti-inflammatory study on crude methanol extract and different fractions of Eremostachys laciniata. Pharmaceutical biology. 2010;48(10):1115-8.
14. Modaressi M, Delazar A, Nazemiyeh H, Fathi‐Azad F, Smith E, Rahman MM, et al. Antibacterial iridoid glucosides from Eremostachys laciniata. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2009;23(1):99-103.
15. Xiong G, Ji W, Wang F, Zhang F, Xue P, Cheng M, et al. Quercetin inhibits inflammatory response induced by LPS from Porphyromonas gingivalis in human gingival fibroblasts via suppressing NF-κB signaling pathway. BioMed research international. 2019;2019.
16. Weinreb M, Nemcovsky CE. In vitro models for evaluation of periodontal wound healing/regeneration. Periodontology 2000. 2015;68(1):41-54.
17. Xiong G, Ji W, Wang F, Zhang F, Xue P, Cheng M, et al. Quercetin inhibits inflammatory response induced by LPS from porphyromonas gingivalis in human gingival fibroblasts via suppressing NF-κB signaling pathway. BioMed research international.2019.
18. Politis C, Schoenaers J, Jacobs R, Agbaje JO. Wound healing problems in the mouth. Frontiers in physiology. 2016;7:507.
19. Nazir MA. Prevalence of periodontal disease, its association with systemic diseases and prevention. International journal of health sciences. 2017;11(2):72.
20. Ahangar P, Mills SJ, Smith LE, Gronthos S, Cowin AJ. Human gingival fibroblast secretome accelerates wound healing through anti-inflammatory and pro-angiogenic mechanisms. NPJ Regenerative medicine. 2020;5(1):1-10.
21. Polimeni G, Xiropaidis AV, Wikesjö UM. Biology and principles of periodontal wound healing/regeneration. Periodontology 2000. 2006;41(1):30-47.
22. Chiquet M, Katsaros C, Kletsas D. Multiple functions of gingival and mucoperiosteal fibroblasts in oral wound healing and repair. Periodontology 2000. 2015;68(1):21-40.
23. Schett G, Dayer J-M, Manger B. Interleukin-1 function and role in rheumatic disease. Nature Reviews Rheumatology. 2016;12(1):14.
24. Cheng R, Wu Z, Li M, Shao M, Hu T. Interleukin-1β is a potential therapeutic target for periodontitis: a narrative review. International journal of oral science. 2020;12(1):1-9.
25. Reinhardt RA, Masada MP, Kaldahl WB, DuBois LM, Kornman KS, Choi JI, et al. Gingival fluid IL‐1 and IL‐6 levels in refractory periodontitis. Journal of clinical periodontology. 1993;20(3):225-31.
26. Kobayashi M, Squires GR, Mousa A, Tanzer M, Zukor DJ, Antoniou J, et al. Role of interleukin‐1 and tumor necrosis factor α in matrix degradation of human osteoarthritic cartilage. Arthritis & Rheumatism: Official Journal of the American College of Rheumatology. 2005;52(1):128-35.
27. Zhang X, Kohli M, Zhou Q, Graves DT, Amar S. Short-and long-term effects of IL-1 and TNF antagonists on periodontal wound healing. The journal of immunology. 2004;173(5):3514-23.
28. Oh H, Hirano J, Takai H, Ogata Y. Effects of initial periodontal therapy on interleukin-1β level in gingival crevicular fluid and clinical periodontal parameters. Journal of oral science. 2015;57(2):67-71.
29. Nathan C. Points of control in inflammation. Nature. 2002;420(6917):846-52.
30. Declercq W, Denecker G, Fiers W, Vandenabeele P. Cooperation of both TNF receptors in inducing apoptosis: involvement of the TNF receptor-associated factor binding domain of the TNF receptor 75. The Journal of Immunology. 1998;161(1):390-9.
31. Xiao W, Hodge DR, Wang L, Yang X, Zhang X, Farrar WL. NF-kappaB activates IL-6 expression through cooperation with c-Jun and IL6-AP1 site, but is independent of its IL6-NFkappaB regulatory site in autocrine human multiple myeloma cells. Cancer biology & therapy. 2004;3(10):1007-17.
32. Johannsen A, Rydmark I, Söder B, Åsberg M. Gingival inflammation, increased periodontal pocket depth and elevated interleukin‐6 in gingival crevicular fluid of depressed women on long‐term sick leave. Journal of periodontal research. 2007;42(6):546-52.
33. Lin S-J, Chen Y-L, Kuo MY-B, Li C-L, Lu H-K. Measurement of gp130 cytokines–Oncostatin M and IL-6 in gingival crevicular fluid of patients with chronic periodontitis. Cytokine. 2005;30(4):160-7.
34. Choi DH, Moon IS, Choi BK, Paik JW, Kim YS, Choi SH, et al. Effects of sub‐antimicrobial dose doxycycline therapy on crevicular fluid MMP‐8, and gingival tissue MMP‐9, TIMP‐1 and IL‐6 levels in chronic periodontitis. Journal of periodontal research. 2004;39(1):20-6.
35. Ancuța C, Chirieac R, Ancuța E, Țănculescu O, Solomon SM, Fătu AM, et al. Exploring the Role of Interleukin-6 Receptor Inhibitor Tocilizumab in Patients with Active Rheumatoid Arthritis and Periodontal Disease. Journal of Clinical Medicine. 2021;10(4):878.
36. Smith PC, Martínez C, Martínez J, McCulloch CA. Role of fibroblast populations in periodontal wound healing and tissue remodeling. Frontiers in physiology. 2019;10:270.
37. Liao J, Azelmat J, Zhao L, Yoshioka M, Hinode D, Grenier D. The Kampo medicine Rokumigan possesses antibiofilm, anti-inflammatory, and wound healing properties. BioMed research international. 2014;2014.
Published
2021-06-26
How to Cite
S.HARIRI, Armin et al. Eremostachys Binalodensis, A Potential Therapeutic Choice for Gingival Inflammatory Wounds. Journal of Contemporary Medical Sciences, [S.l.], v. 7, n. 3, june 2021. ISSN 2413-0516. Available at: <http://www.jocms.org/index.php/jcms/article/view/976>. Date accessed: 16 sep. 2021. doi: https://doi.org/10.22317/jcms.v7i3.976.
Section
Articles