Evaluation of the Cytotoxic Activity of Anthriscus nemorosa on Breast Cancer Cells

  • Haleh Forouhandeh Molecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Mahtab Zarchini Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Elham Safarzadeh Department of Microbiology & Immunology, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
  • Omoleila Molavi Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Parina Asgharian Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. http://orcid.org/0000-0001-9110-8209
  • Vahideh Tarhriz Molecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran. http://orcid.org/0000-0002-3018-9313

Abstract

Objective: The present study concerns the cytotoxic activity of A. nemorosa different extracts on breast cancer cells  (MCF-7) and normal cell lines (HFFF).
Methods: Different extracts of aerial parts of  A. nemorosa were prepared using Soxhlet apparatus. The cytotoxicity of samples was assessed by MTT assay on breast cancer cells (MCF-7) and noncancerous cells (HFFF) with different concentrations of extracts in 24 and 48 hours. The most potent extract was fractioned and cytotoxic activity of fractions was considered, As well. A flow cytometry (annexin V/PI) assay has been used for detecting the mechanism of cell death in sample treated cell lines. Moreover, for clarifying volatile components of n-Hexane extract and its 80% and 100% VLC fractions were subjected to GC-MS apparatus.
Results: Results indicated that n-Hexane extract and its 80% and 100% VLC fractions exhibited a significant (p<0.001) inhibitory effect on the growth of the MCF-7 cell line compared to the control group. Meanwhile, flow cytometry analysis revealed that potent extract caused cell death through necrosis and 80% and 100% fractions showed different mechanisms (such as autophagy). The major compounds, which maybe were in charge of showing cytotoxic activity were non-terpenoids.
Conclusion: This study provides the evidence that in vitro cytotoxic activity of n-Hexane extract and 80% and 100% VLC fractions of A. nemorosa inhibited the proliferation of breast cancer cells (MCF7) via a different mechanism.
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Published
2021-08-26
How to Cite
FOROUHANDEH, Haleh et al. Evaluation of the Cytotoxic Activity of Anthriscus nemorosa on Breast Cancer Cells. Journal of Contemporary Medical Sciences, [S.l.], v. 7, n. 4, p. 217-226, aug. 2021. ISSN 2413-0516. Available at: <http://www.jocms.org/index.php/jcms/article/view/940>. Date accessed: 02 dec. 2021. doi: https://doi.org/10.22317/jcms.v7i2.940.