LEP and LEPR Polymorphisms Influences Anthropometric Outcome in Response to 8 Weeks of Combined Training in Obese boys
AbstractObjectives: The purpose of the present study was to investigate whether LEP19 G>A and LEPR 668 A>G polymorphisms, would influence the effect of an 8-week combined aerobic and resistance training.
Methods: Thirty obese boys (BMIz>+2) aged 11-13 (12.66±0.47) were recruited from three middle schools in Quchan. The changes in body composition parameters and metabolic factors in response to 8-weeks combined aerobic and resistance training program were analyzed regarding LEP and LEPR polymorphism. DNA was extracted from cheek cells donated by the 30 participants and genotyping was carried out using PCR.
Results: Our results suggest that carriers of rs2167270G allele and rs1137101A allele were characterized by a greater reduction in body mass and WHR (P< 0.05). Also, a significant decrease was observed in leptin levels in carriers of rs2167270G allele after the training program (P=0.031). Moreover, the LEP and LEPR polymorphisms were associated with changes in lipid profile in response to training.
Conclusion: In response to 8 weeks of regular physical activity, obese boys with G (rs2167270) and A (rs1137101) alleles had the best likelihood of losing weight which was associated with a decrease in body mass, fat mass (%), WHR and leptin concentrations.
2. Ng M, Fleming T, Robinson M, Thomson B, Graetz N, Margono C, et al. Global, regional, and national prevalence of overweight and obesity in children and adults during 1980–2013: a systematic analysis for the Global Burden of Disease Study 2013. The lancet. 2014;384(9945):766-81.
3. Farrag NS, Cheskin LJ, Farag MK. A systematic review of childhood obesity in the Middle East and North Africa (MENA) region: Prevalence and risk factors meta-analysis. Advances in pediatric research. 2017;4.
4. Samaras K, Kelly PJ, Chiano MN, Spector TD, Campbell LV. Genetic and environmental influences on total-body and central abdominal fat: the effect of physical activity in female twins. Annals of Internal Medicine. 1999;130(11):873-82.
5. Kelishadi R. Childhood overweight, obesity, and the metabolic syndrome in developing countries. Epidemiologic reviews. 2007;29(1):62-76.
6. Rahmanian M, Kelishadi R, Qorbani M, Motlagh ME, Shafiee G, Aminaee T, et al. Dual burden of body weight among Iranian children and adolescents in 2003 and 2010: the CASPIAN-III study. Archives of medical science: AMS. 2014;10(1):96.
7. Wang K, Li WD, Zhang CK, Wang Z, Glessner JT, Grant SF, et al. A genome-wide association study on obesity and obesity-related traits. PloS one. 2011;6(4):e18939.
8. Mărginean CO, Mărginean C, Voidăzan S, Meliţ L, Crauciuc A, Duicu C, et al. Correlations between leptin gene polymorphisms 223 A/G, 1019 G/A, 492 G/C, 976 C/A, and anthropometrical and biochemical parameters in children with obesity: a prospective case-control study in a Romanian population—the nutrichild study. Medicine. 2016;95(12).
9. Lakshman R, Elks CE, Ong KK. Childhood obesity. Circulation. 2012;126(14):1770-9.
10. Walsh S, Haddad C, Kostek M, Angelopoulos T, Clarkson P, Gordon P, et al. Leptin and leptin receptor genetic variants associate with habitual physical activity and the arm body composition response to resistance training. Gene. 2012;510(1):66-70.
11. Lakka TA, Rankinen T, Weisnagel SJ, Chagnon YC, Lakka H-M, Ukkola O, et al. Leptin and leptin receptor gene polymorphisms and changes in glucose homeostasis in response to regular exercise in nondiabetic individuals: the HERITAGE family study. Diabetes. 2004;53(6):1603-8.
12. Leonska-Duniec A, Jastrzebski Z, Jazdzewska A, Krzysztof F, Cieszczyk P. Leptin and leptin receptor genes are associated with obesity-related traits changes in response to aerobic training program. The Journal of Strength & Conditioning Research. 2018;32(4):1036-44.
13. Hager J, Clement K, Francke S, Dina C, Raison J, Lahlou N, et al. A polymorphism in the 5′ untranslated region of the human ob gene is associated with low leptin levels. International journal of obesity. 1998;22(3):200-5.
14. Enns JE, Taylor CG, Zahradka P. Variations in adipokine genes AdipoQ, Lep, and LepR are associated with risk for obesity-related metabolic disease: the modulatory role of gene-nutrient interactions. Journal of obesity. 2011;2011.
15. Bruce Thompson D, Ravussin E, Bennett PH, Bogardus C. Structure and sequence variation at the human leptin receptor gene in lean and obese Pima Indians. Human molecular genetics. 1997;6(5):675-9.
16. Paracchini V, Pedotti P, Taioli E. Genetics of leptin and obesity: a HuGE review. American journal of epidemiology. 2005;162(2):101-14.
17. Chagnon YC, Wilmore JH, Borecki IB, Gagnon J, Pérusse L, Chagnon M, et al. Associations between the leptin receptor gene and adiposity in middle-aged Caucasian males from the HERITAGE family study. The Journal of Clinical Endocrinology & Metabolism. 2000;85(1):29-34.
18. Ogawa T, Hirose H, Yamamoto Y, Nishikai K, Miyashita K, Nakamura H, et al. Relationships between serum soluble leptin receptor level and serum leptin and adiponectin levels, insulin resistance index, lipid profile, and leptin receptor gene polymorphisms in the Japanese population. Metabolism. 2004;53(7):879-85.
19. Portolés O, Sorlí JV, Francés F, Coltell O, González JI, Sáiz C, et al. Effect of genetic variation in the leptin gene promoter and the leptin receptor gene on obesity risk in a population-based case-control study in Spain. European journal of epidemiology. 2006;21(8):605-12.
20. Guizar-Mendoza J, Amador-Licona N, Flores-Martinez S, Lopez-Cardona M, Ahuatzin-Tremary R, Sanchez-Corona J. Association analysis of the Gln223Arg polymorphism in the human leptin receptor gene, and traits related to obesity in Mexican adolescents. Journal of human hypertension. 2005;19(5):341-6.
21. Bouchard C. Gene–environment interactions in the etiology of obesity: defining the fundamentals. Obesity. 2008;16(S3):S5-S10.
22. Ordovas JM, Shen J. Gene–environment interactions and susceptibility to metabolic syndrome and other chronic diseases. Journal of periodontology. 2008;79:1508-13.
23. Gajewska J, Kurylowicz A, Ambroszkiewicz J, Mierzejewska E, Chelchowska M, Szamotulska K, et al. ADIPOQ− 11377C> G polymorphism increases the risk of adipokine abnormalities and child obesity regardless of dietary intake. Journal of pediatric gastroenterology and nutrition. 2016;62(1):122-9.
24. Alberga AS, Prud'homme D, Sigal RJ, Goldfield GS, Hadjiyannakis S, Phillips P, et al. Effects of aerobic training, resistance training, or both on cardiorespiratory and musculoskeletal fitness in adolescents with obesity: the HEARTY trial. Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme. 2016;41(3):255-65.
25. Marshall WA, Tanner JM. Variations in the pattern of pubertal changes in boys. Archives of disease in childhood. 1970;45(239):13-23.
26. Ho M, Garnett SP, Baur L, Burrows T, Stewart L, Neve M, et al. Effectiveness of lifestyle interventions in child obesity: systematic review with meta-analysis. Pediatrics. 2012;130(6):e1647-e71.
27. Poitou C, Lacorte J-M, Coupaye M, Bertrais S, Bedel J-F, Lafon N, et al. Relationship between single nucleotide polymorphisms in leptin, IL6 and adiponectin genes and their circulating product in morbidly obese subjects before and after gastric banding surgery. Obesity Surgery. 2005;15(1):11-23.
28. Jiang Y, Wilk J, Borecki I, Williamson S, DeStefano A, Xu G, et al. Common variants in the 5′ region of the leptin gene are associated with body mass index in men from the National Heart, Lung, and Blood Institute Family Heart Study. The American Journal of Human Genetics. 2004;75(2):220-30.
29. Furusawa T, Naka I, Yamauchi T, Natsuhara K, Kimura R, Nakazawa M, et al. The serum leptin level and body mass index in Melanesian and Micronesian Solomon Islanders: focus on genetic factors and urbanization. American Journal of Human Biology. 2011;23(4):435-44.
30. Ali SB, Kallel A, Sediri Y, Ftouhi B, Feki M, Slimene H, et al. LEPR p. Q223R Polymorphism influences plasma leptin levels and body mass index in Tunisian obese patients. Archives of medical research. 2009;40(3):186-90.
31. Suriyaprom K, Tungtrongchitr R, Thawnasom K. Measurement of the levels of leptin, BDNF associated with polymorphisms LEP G2548A, LEPR Gln223Arg and BDNF Val66Met in Thai with metabolic syndrome. Diabetology & metabolic syndrome. 2014;6(1):6.
32. Oliveira Rd, Cerda A, Genvigir FDV, Sampaio MF, Armaganijan D, Bernik MMS, et al. Leptin receptor gene polymorphisms are associated with adiposity and metabolic alterations in Brazilian individuals. Arquivos Brasileiros de endocrinologia & metabologia. 2013;57(9):677-84.
33. VanPatten S, Ranginani N, Shefer S, Nguyen LB, Rossetti L, Cohen DE. Impaired biliary lipid secretion in obese Zucker rats: leptin promotes hepatic cholesterol clearance. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2001;281(2):G393-G404.
34. Takahashi-Yasuno A, Masuzaki H, Miyawaki T, Ogawa Y, Matsuoka N, Hayashi T, et al. Leptin receptor polymorphism is associated with serum lipid levels and impairment of cholesterol lowering effect by simvastatin in Japanese men. Diabetes research and clinical practice. 2003;62(3):169-75.
35. Shabana N, Hasnain S. Association of the leptin receptor Gln223 Arg polymorphism with lipid profile in obese Pakistani subjects. Nutrition. 2015;31(9):1136-40.
36. Becer E, Mehmetçik G, Bareke H, Serakıncı N. Association of leptin receptor gene Q223R polymorphism on lipid profiles in comparison study between obese and non-obese subjects. Gene. 2013;529(1):16-20.
37. Daghestani M, Purohit R, Daghestani M, Daghistani M, Warsy A. Molecular dynamic (MD) studies on Gln233Arg (rs1137101) polymorphism of leptin receptor gene and associated variations in the anthropometric and metabolic profiles of Saudi women. PloS one. 2019;14(2).
38. Steinberg GR, Smith AC, Wormald S, Malenfant P, Collier C, Dyck DJ. Endurance training partially reverses dietary-induced leptin resistance in rodent skeletal muscle. American Journal of Physiology-Endocrinology And Metabolism. 2004;286(1):E57-E63.
39. Steinberg GR, Rush JW, Dyck DJ. AMPK expression and phosphorylation are increased in rodent muscle after chronic leptin treatment. American Journal of Physiology-Endocrinology and Metabolism. 2003;284(3):E648-E54.
40. De Araujo ACC, Roschel H, Picanço AR, do Prado DML, Villares SMF, de Sá Pinto AL, et al. Similar health benefits of endurance and high-intensity interval training in obese children. PloS one. 2012;7(8).
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.