Evaluation of the effectiveness and safety of N-Chromosome Royal Jelly on the number of peripheral white blood cells in patients with acute lymphoblastic leukemia

  • Gholamreza Bahoush 1. Department of Pediatric Hematology and Oncology, Ali-Asghar children`s Hospital, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Rozhin Pahlevani 1. Department of Pediatric Hematology and Oncology, Ali-Asghar children`s Hospital, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.


Objectives: This study to investigate the immune response blood cells following N-Chromosome RJ administration in patients with childhood ALL.
Methods: This single center before and after 12-weeks clinical trial was done in Ali-Asghar Children Hospital on twenty-six patients diagnosed with ALL during maintenance phase of chemotherapy. After collecting their demographics, patients received a starter dose of 2-gram processed new natural   N-chromosome RJ before breakfast and were followed up each 2 weeks, counting their peripheral WBC, ANC and ALC by a blood cell count and differential analysis.
Results: A total of Twenty-six patients were enrolled in this study (16 males and 10 females). Mean Peripheral WBC count of total patients were raised significantly after administering N-RJ Being 2510 ±1192 cells per cubic millimeter at the beginning and then raised to 4549±1500 cells per cubic millimeter at the end of trial. (P<0.005) None of patients suffered from any adverse reaction during the trial.
There was a positive statistical relationship between total peripheral WBC, ANC and ALC count and N-Chromosome Royal jelly increased dosage, Being more prominent at the beginning of trial (p<0.001) and the last two weeks of follow-ups. (p<0.0005)
Conclusion: This study has successfully demonstrated that N-chromosome RJ can be a promising immune- enhancing supplement in patients diagnosed with acute lymphoblastic leukemia in their complete remission and maintenance therapy time. In addition, it is a natural alternative for drugs like G-CSF, but without those long-term adverse effects, we can see in G-CSF drugs.
share this Article by


1. Horibe K, Saito AM, Takimoto T, Tsuchida M, Manabe A, Shima M, et al. Incidence and survival rates of hematological malignancies in Japanese children and adolescents (2006–2010): based on registry data from the Japanese Society of Pediatric Hematology. International journal of hematology. 2013;98(1):74-88.
2. Inaba H, Greaves M, Mullighan CG. Acute lymphoblastic leukaemia. The Lancet. 2013;381(9881):1943-55.
3. Schmiegelow K, Attarbaschi A, Barzilai S, Escherich G, Frandsen TL, Halsey C, et al. Consensus definitions of 14 severe acute toxic effects for childhood lymphoblastic leukaemia treatment: a Delphi consensus. The Lancet Oncology. 2016;17(6):e231-e9.
4. Kato M. Pediatric Acute Lymphoblastic Leukemia: Springer; 2020.
5. Lieschke GJ, Burgess AW. Granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor. New England Journal of Medicine. 1992;327(1):28-35.
6. Wang Y, Chen L, Liu F, Zhao N, Xu L, Fu B, et al. Efficacy and tolerability of granulocyte colony-stimulating factors in cancer patients after chemotherapy: A systematic review and Bayesian network meta-analysis. Scientific reports. 2019;9(1):1-12.
7. Pfeil AM, Allcott K, Pettengell R, von Minckwitz G, Schwenkglenks M, Szabo Z. Efficacy, effectiveness and safety of long-acting granulocyte colony-stimulating factors for prophylaxis of chemotherapy-induced neutropenia in patients with cancer: a systematic review. Supportive care in cancer. 2015; 23(2):525-45.
8. Bronchud MH‚ Scarffe JH‚ Thatcher N‚ et al. Phase I/II study of recombinant human granulocyte colony-stimulating factor in patients receiving intensive chemotherapy for small cell lung cancer. Br J Cancer. 1987; 56:809-813.
9. Heil G, Hoelzer D, Sanz MA, et al. A randomized, double-blind, placebo-controlled, phase III study of filgrastim in remission induction and consolidation therapy for adults with de novo Acute Myeloid Leukemia. Blood. 1997; 90:4710-4718.
10. Dale DC‚ Bonilla MA‚ Davis MW‚ et al. A randomized controlled phase III trial of recombinant human granulocyte colony-stimulating factor (filgrastim) for treatment of severe chronic neutropenia. Blood. 1993; 81:2496-2502.
11. HIGHLIGHTS OF PRESCRIBING INFORMATION: These highlights do not include all the information needed to use NEUPOGEN safely and effectively; Initial FDA Approval: 1991.
12. Kaftanoglu O, Tanyeli A. The use of royal jelly during treatment of childhood malignancies. Bee Products: Springer; 1997. p. 179-83.
13. Gasic S, Vucevic D, Vasilijic S, Antunovic M, Chinou I, Colic M. Evaluation of the immunomodulatory activities of royal jelly components in vitro. Immunopharmacology and Immunotoxicology. 2007;29(3-4):521-36.
14. Mirabzadeh A. The use of N-chromosome Royal Jelly to treat H. InPilory Ulcers. Abstract-XXXXIII International Apicultural Congress (Vol. 29, pp. 76-77).
15. Smith MA, Seibel NL, Altekruse SF, Ries LA, Melbert DL, O'Leary M, et al. Outcomes for children and adolescents with cancer: challenges for the twenty-first century. Journal of clinical oncology. 2010;28(15):2625.
16. Kliegman RM SB, Geme JSt, Schor NF. Nelson, ed. t. Textbook of Pediatrics(Chapter 495): Philadelphia: Elsevier; ; 2016.
17. Badr M, Hassan T, Sakr H, Karam N, Rahman DA, Shahbah D, et al. Chemotherapy‑induced neutropenia among pediatric cancer patients in Egypt: Risks and consequences. Mol Clin Oncol. 2016;5(3):300-6.
18. Ozer H, Armitage JO, Bennett CL, Crawford J, Demetri GD, Pizzo PA, et al. 2000 update of recommendations for the use of hematopoietic colony-stimulating factors: evidence-based, clinical practice guidelines. American Society of Clinical Oncology Growth Factors Expert Panel. Journal of clinical oncology: official journal of the American Society of Clinical Oncology. 2000;18(20):3558.
19. Zahran AM, Elsayh KI, Saad K, Eloseily EM, Osman NS, Alblihed MA, et al. Effects of royal jelly supplementation on regulatory T cells in children with SLE. Food & nutrition research. 2016;60:32963.
20. Shirzad M, Kordyazdi R, Shahinfard N, Nikokar M. Does Royal jelly affect tumor cells? Journal of HerbMed Pharmacology. 2013;2(2).
21. Gismondi A, Trionfera E, Canuti L, Di Marco G, Canini A. Royal jelly lipophilic fraction induces antiproliferative effects on SH-SY5Y human neuroblastoma cells. Oncology Reports. 2017;38(3):1833-44.
How to Cite
BAHOUSH, Gholamreza; PAHLEVANI, Rozhin. Evaluation of the effectiveness and safety of N-Chromosome Royal Jelly on the number of peripheral white blood cells in patients with acute lymphoblastic leukemia. Journal of Contemporary Medical Sciences, [S.l.], v. 6, n. 6, dec. 2020. ISSN 2413-0516. Available at: <http://www.jocms.org/index.php/jcms/article/view/887>. Date accessed: 14 apr. 2021. doi: https://doi.org/10.22317/jcms.v6i6.887.