Evaluation of T-Bet immunostaining in the counting of intraepithelial lymphocytes in celiac disease

  • Amna E. Al-araji Department of Pathological Analysis, University of Kafeel, Al-Najaf, Iraq.
  • Tuqa Z. Omran Department of Medical sciences, University of Al-Ameed, Kerbala, Iraq.
  • Mohanad M. Ahmed Department of medical microbiology and immunology, Kerbala University, Kerbala, Iraq.
  • Nazar J. Metib Consulting Pathologist, Al-Hussein Medical City, Kerbala, Iraq.


Objective: In this study, we aimed to evaluate CD3, T-bet and GATA3 staining of intraepithelial lymphocytes (IELs) in comparison to the routine H&E stains in celiac disease.
Methods: a total of 50 patients, in whom celiac disease was diagnosed based on a combination of clinicopathological features, were enrolled in the study. Duodenal biopsied tissues were processed routinely into formalin fixed paraffin embedded (FFPE) blocks. Sections were prepared and stained with H&E and each of CD3, T-bet and GATA3. A number of histological criteria were measured to calculate the Marsh score. The results were analyzed using the IBM SPSS analytic software.
Results: a positive correlation was found between the count of T-bet stained cells and the increase of intraepithelial lymphocytes (P-value = 0.001). In addition, low count of GATA3 stained cells was seen in almost all cases. The count of GATA3 stained cells was not affected by the increase in IELs count.
Conclusions: the majority of increased IELs were stained with T-bet. Whereas in normal IELs count is less than half the IELs were stained with T-bet. This would indicate that T-bet immunostaining is a potential alternative to H&E and/or CD3 based counting of IELs.
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How to Cite
AL-ARAJI, Amna E. et al. Evaluation of T-Bet immunostaining in the counting of intraepithelial lymphocytes in celiac disease. Journal of Contemporary Medical Sciences, [S.l.], v. 6, n. 5, oct. 2020. ISSN 2413-0516. Available at: <http://www.jocms.org/index.php/jcms/article/view/861>. Date accessed: 25 nov. 2020. doi: https://doi.org/10.22317/jcms.v6i5.861.