Investigating the healing effect of the hydroalcoholic extract of pomegranate seed (Punica granatum) on the full thickness wound in rabbit

  • Ali Asghar Hemmati Departmant of Pharmacology, School of Pharmacy, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran.
  • Iran Rashidi Department of Pathology, School of Medicine, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran.
  • Sedigheh Dahanzadeh Departmant of Pharmacology, School of Pharmacy, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran
  • Mahmoud Moeini Departmant of Pharmacology, School of Pharmacy, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran.


Objective: This research designed to investigate the wound healing process with pomegranate hydroalcoholic seed extract (Punica granatum) in comparison with no-treatment, betamethasone, phenytoin and eucerin in rabbits.
Methods: The positive group including groups that received phenytoin cream (1%) and topical eucerin, respectively, twice a day to complete wound healing. negative Control group did not obtain any treatment. Treatment groups were received cream of PSE (2, 5,7,10 w/w) in eucerin and 75% w/w as purified extract twice daily. In order to measure the percentage of wound healing, the zone of the wound was evaluated daily. Histological studies were done on the 7th and 15th days of treatment. Next, hydroxyproline content of wounds healed and tensile strength of wound tissue samples were measured.
Results: The results demonstrated between PSE treatment groups and eucerin animals were statistically significant aadifferences (P<0.05) in most of the days reviewed. Treatment of Rabbits with 10% PSE had the best results (complete wound recovery in 12 days). Also, this treatment showed higher hydroxyproline content and higher tissue strength.
Conclusion: This research reveals that the extract of 10% PSE administrated topically has the proper potential to induce wound recovery in the wound model of rabbits. In addition, 10% PSE accelerates the healing of the wound. Further study needs to clarify the results of this research.
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How to Cite
HEMMATI, Ali Asghar et al. Investigating the healing effect of the hydroalcoholic extract of pomegranate seed (Punica granatum) on the full thickness wound in rabbit. Journal of Contemporary Medical Sciences, [S.l.], v. 6, n. 2, apr. 2020. ISSN 2413-0516. Available at: <>. Date accessed: 14 july 2020. doi: