Maternal separation can affect the reproductive system by inflammasome activation in female mice

  • Kajal Khodamoradi Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Hossein Amini-Khoei Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
  • Zahra Khosravizadeh Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Seyed Reza Hosseini Departent of Urology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Ahmad Reza Dehpour Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran
  • Gholamreza Hassanzadeh Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Objective: The aim of this study is to investigate effect of maternal separation stress on the ovarian function in adult female mice.  
Methods: In this study, maternal separation in pups was performed during post-natal days (PND) 2 to 14. The histological alterations in ovarian tissue, ROS production (using DCFH-DA assay), gene expression of NLRP3, ASC, caspase-1, TLR4, BAX, BCL-2 and TNFα (using RT-PCR), protein levels of ATP, GPx, IL-1β and IL-18 (using ELISA). Also, protein expression of caspase-3 and NLRP3 (using immunocytochemistry) were evaluated.
Results: Results showed that maternal separation decreased percentage of primordial follicles while increased percentage of secondary and graafian follicles. In addition, maternal separation increased ROS production and decreased ATP and GPx concentrations. Furthermore, maternal separation significantly affected expression of cytokines and genes involved in inflammation and apoptosis including NLRP3, ASC, caspase-1, TLR4, TNFα, IL-1β, IL-18 BAX and BCL2. Findings also showed that stress-induced maternal separation significantly increased percentage of caspase-3 and NLRP3 positive cells. We concluded that maternal separation stress has harmful effects on ovarian tissue.
Conclusion: It seems that these harmful effects are probably occur through increase of ROS production and impact on mitochondrial function, inflammatory process and apoptosis pathways.
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Published
2019-06-28
How to Cite
KHODAMORADI, Kajal et al. Maternal separation can affect the reproductive system by inflammasome activation in female mice. Journal of Contemporary Medical Sciences, [S.l.], v. 5, n. 3, june 2019. ISSN 2413-0516. Available at: <http://www.jocms.org/index.php/jcms/article/view/601>. Date accessed: 21 nov. 2019.
Section
Articles

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