Production and optimisation of hyaluronic acid extracted from Streptococcus pyogenes

  • Kawkab Abdulla Al-Saadiaa University of Karbala, College of Science, Department of Biology
  • Hassan Fadhil Naji University of Babylon, College of Science, Department of Biology
  • Ali Hmood Al-Saadi University of Babylon, College of Science, Department of Biology

Abstract

Objectives Because of the less studies in this field in Iraq, this study aimed to use local Streptococcus pyogenes isolates to produce hyluronic acid.Methods The quantitative estimation of hyaluronic acid (HA) produced from eight local S. pyogenes isolates at different pH (6.3, 6.6, 6.9, 7.2,and 7.5) and glucose concentration (4%, 6%, 8%, and 1%) were done using the HA ELISA kit.Results This study showed that the maximum yield of HA was obtained at pH 7.5, and it was found that the differences in pH of theproduction media of HA enhanced the HA production, but the glucose concentration has no beneficial effect for HA production.Conclusion There were differences in HA production among local isolates at the same pH.
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Published
2016-01-08
How to Cite
AL-SAADIAA, Kawkab Abdulla; NAJI, Hassan Fadhil; AL-SAADI, Ali Hmood. Production and optimisation of hyaluronic acid extracted from Streptococcus pyogenes. Journal of Contemporary Medical Sciences, [S.l.], v. 1, n. 4, p. 13-15, jan. 2016. ISSN 2413-0516. Available at: <http://www.jocms.org/index.php/jcms/article/view/52>. Date accessed: 30 may 2017.
Section
Articles

Keywords

Streptococcus pyogenes, hyaluronic acid, HA ELISA kit, pH, glucose concentration