Production and optimisation of hyaluronic acid extracted from Streptococcus pyogenes
AbstractObjectives Because of the less studies in this field in Iraq, this study aimed to use local Streptococcus pyogenes isolates to produce hyluronic acid.Methods The quantitative estimation of hyaluronic acid (HA) produced from eight local S. pyogenes isolates at different pH (6.3, 6.6, 6.9, 7.2,and 7.5) and glucose concentration (4%, 6%, 8%, and 1%) were done using the HA ELISA kit.Results This study showed that the maximum yield of HA was obtained at pH 7.5, and it was found that the differences in pH of theproduction media of HA enhanced the HA production, but the glucose concentration has no beneficial effect for HA production.Conclusion There were differences in HA production among local isolates at the same pH.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.Copyright of the manuscripts is retained by the authors. Authors grant Nab'a Al-Hayat Foundation for Medical Sciences and Health Care - Press, an exclusive unlimited license to publish the article under a Creative Commons Attribution-NonCommercial 3.0 and identify Nab'a Al-Hayat Foundation for Medical Sciences and Health Care - Press as the sole publisher. Submission of an article for publication implies the authors’ consent to publication under the applicable Creative Commons license and the terms and conditions of the Publisher’s Licensing Agreement.