Evaluation the Effect of Royal Jelly on the Growth of Two Members of Gut Microbiota; Bacteroides fragillis and Bacteroides thetaiotaomicron

Authors

  • Vida Kazemi Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Mojtaba Mojtahedzadeh Department of Pharmacotherapy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Seyed Davar Siadat Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
  • Abbas Hadjiakhondi Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Abdulghani Ameri Department of food and drug control, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
  • Sara Ahmadi Badi Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
  • Azadeh Manayi Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Mahdi Bagheri Department of Pharmacotherapy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

DOI:

https://doi.org/10.22317/jcms.v5i1.518

Keywords:

Bacteroides fragile, Bacteroides thetaiotaomicron, Gastrointestinal tract microbiota, Gut microbiota, Royal jelly, Traditional medicine

Abstract

Objective: In this study the effect of Royal jelly on the growth of two important members of Bacteroides spp.; Bacteroides fragilis and Bacteroides thetaiotaomicron, was evaluated. Also the physicochemical properties and cytotoxicity effects of Royal jelly on Caco-2 cell line as gastrointestinal epithelial cell model, assessed.

Methods : Bacteria, Bacteroides fragilis and Bacteroides thetaiotaomicron were grown on brain heart infusion (BHI) broth medium supplemented with Royal jelly in 3 different concentrations (2.5, 5 and 10% v/v), both of the bacteria (1.5×108 cfu/mL) were inoculated to BHI broth contained Royal jelly in anaerobic condition. To calculate the bacterial optical density (OD), the absorbance was measured at 600 nm after an overnight. Also Caco-2 cells, was used to study the effects of Royal jelly on epithelial cell viability, and the Physicochemical properties consist of  total proteins, polysaccharides, phenolic compounds, total lipids, ash and moisture by UV-VIS spectrophotometric and gravimetric methods were evaluated .

Results: The growth of B. fragillis and B. thetaiotaomicron were increased by Royal jelly (2.5, 5 and 10% v/v concentrations) and the results indicated that Royal jelly increased the growth of bacteria in a dose dependent manner (p<0.001). In addition MTT assay showed more than 95% viability of Caco-2 cells treated with Royal jelly. The Iranian Royal jelly sample contains 59.01% water, 11.57% proteins, 12% lipids, 12.63 % polysaccharide and 5% mineral.

Conclusion: The present study showed that Royal jelly has a potential effect in the  preserving gut microbiota  and it is suggested that Royal jelly as a complementary and alternative medicine can be used to treatment diseases are associated  with gut microbiota- host interactions and immune regulating. Although we need to expand our knowledge by designing clinical trials to conï¬rm the therapeutic effects of Royal jelly on gut microbiota modulation as a barrier function.

References

1. Alvarez-Suarez, J.M., et al., Contribution of honey in nutrition and human health: a review. Mediterranean Journal of Nutrition and Metabolism, 2010. 3(1): p. 15-23.
2. Pasupuleti, V.R., et al., Honey, propolis, and royal jelly: a comprehensive review of their biological actions and health benefits. Oxidative medicine and cellular longevity, 2017. 2017.
3. Rao, P.V., et al., Biological and therapeutic effects of honey produced by honey bees and stingless bees: a comparative review. Revista Brasileira de Farmacognosia, 2016. 26(5): p. 657-664.
4. Benson, A.K., et al., Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors. Proceedings of the National Academy of Sciences, 2010. 107(44): p. 18933-18938.
5. Clemente, J.C., et al., The impact of the gut microbiota on human health: an integrative view. Cell, 2012. 148(6): p. 1258-1270.
6. Lozupone, C.A., et al., Diversity, stability and resilience of the human gut microbiota. Nature, 2012. 489(7415): p. 220.
7. Sekirov, I., et al., Gut microbiota in health and disease. Physiological reviews, 2010. 90(3): p. 859-904.

8. Nicholson, J.K., et al., Host-gut microbiota metabolic interactions. Science, 2012: p. 1223813.
9. Prakash, S., et al., The gut microbiota and human health with an emphasis on the use of microencapsulated bacterial cells. BioMed Research International, 2011.
10. den Besten, G., et al., The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism. Journal of lipid research, 2013. 54(9): p. 2325-2340.
11. Tremaroli, V. and F. Bäckhed, Functional interactions between the gut microbiota and host metabolism. Nature, 2012. 489(7415): p. 242.
12. Hooper, L.V., D.R. Littman, and A.J. Macpherson, Interactions between the microbiota and the immune system. Science, 2012. 336(6086): p. 1268-1273.
13. Lin, L. and J. Zhang, Role of intestinal microbiota and metabolites on gut homeostasis and human diseases. BMC immunology, 2017. 18(1): p. 2.
14. Tremaroli, V. and F. Bäckhed, Functional interactions between the gut microbiota and host metabolism. Nature, 2012. 489(7415): p. 242-249.

15. Ahmadi Badi S, Siadat SD, Khatami S, Irani S. Induction Effect of Bacteroides fragilis
Derived Outer Membrane Vesicles on Toll Like Receptors Gene Expression and Cytokine Concentrations in Human Intestinal Epithelial Cell. Cell J (Yakhteh). 2019;21(1)


16. Elhenawy, W., M.O. Debelyy, and M.F. Feldman, Preferential packing of acidic glycosidases and proteases into Bacteroides outer membrane vesicles. MBio, 2014. 5(2): p. e00909-14.
17. Ahmed WM, Khalaf AA, Moselhy WA, Safwat GM. Royal jelly attenuates azathioprine induced toxicity in rats. Environ Toxicol Pharmacol. 2014 Jan;37(1):431-7.
18. Özan F, Çörekçi B, Toptaş O, Halicioğlu K, Irgin C, Yilmaz F, Hezenci Y. Effect of Royal Jelly on new bone formation in rapid maxillary expansion in rats. Med Oral Patol Oral Cir Bucal. 2015 Nov 1;20(6):e651-6.
19. Metwally Ibrahim SEL, Kosba AA.Royal jelly supplementation reduces skeletal muscle lipotoxicity and insulin resistance in aged obese rats. Pathophysiology. 2018 Jun 12. pii: S0928-4680(18)30011-7.
20. Boger MCL, Lammerts van Bueren A, Dijkhuizen L.Cross-feeding amongst probiotic bacterial strains on prebiotic inulin involving the extracellular exo-inulinase of Lactobacillus paracasei strain W20. Appl Environ Microbiol. 2018 Aug 31. pii: AEM.01539-18.
21. Melok AL, Lee LH, Mohamed Yussof SA, Chu T. Green Tea Polyphenol Epigallocatechin-3-Gallate-Stearate Inhibits the Growth of Streptococcus mutans: A Promising New Approach in Caries Prevention. Dent J (Basel). 2018 Aug 6;6(3). pii: E38.
22. Mayta-Apaza AC, Pottgen E, De Bodt J, Papp N, Marasini D, Howard L, Abranko L, Van de Wiele T, Lee SO, Carbonero F. Impact of tart cherries polyphenols on the human gut microbiota and phenolic metabolites in vitro and in vivo. J Nutr Biochem. 2018 Apr 7;59:160-172.

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Published

2019-02-26

How to Cite

Kazemi, V., Mojtahedzadeh, M., Siadat, S. D., Hadjiakhondi, A., Ameri, A., Ahmadi Badi, S., Manayi, A., & Bagheri, M. (2019). Evaluation the Effect of Royal Jelly on the Growth of Two Members of Gut Microbiota; Bacteroides fragillis and Bacteroides thetaiotaomicron. Journal of Contemporary Medical Sciences, 5(1), 20–23. https://doi.org/10.22317/jcms.v5i1.518

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