Effects of Genistein Treatment on Molecular Markers Related to Fibrosis: Addition to Histological Changes in the Lung of Ovariectomized Diabetic Rats
AbstractPostmenopausal women experience physiological changes in several organs related to estrogen deficiency. On the other hand, the role of estrogen, as a sex hormone, in the physiological function and structure of the respiratory system has been established. In the present study, we hypothesized that the administration of genistein could reverse lung fibrosis disturbed by estrogen deprivation.
Forty female sprague–dawley rats were assigned into four groups: rats underwent surgery without ovariectomy (sham), rats that underwent ovariectomies (OVX), ovariectomized rats that were fed with high-fat diet (OVX.Dia), ovariectomized diabetic rats with genistein administration (OVX.Dia.Gen). Western blotting technique and hematoxylin & eosin were employed to assess of protein levels and histological changes in the lung.
Transforming growth factor beta-1 and matrix metalloproteinase-2 were found to have a differential expression among the groups. Ovariectomy alone and with diabetes increased the levels of growth factor beta-1 and matrix metalloproteinase-2 as compared with the sham (P<0.05). On the other hand, genistein treatment reversed ovariectomy-induced changes in the protein levels as compared with OVX.Dia group (P<0.05).
Genistein ameliorated expression levels of fibrotic biomarkers in the lung of ovariectomized diabetic rats. Our data suggest that genistein may be beneficial for menopausal women through modulation of pulmonary fibrotic biomarkers expression levels.
Key words: Genistein, Menopause, Lung, Fibrosis, TGFβ1
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