Effect of nutritional factors and growth conditions on biosurfactant production by Pseudomonas mendocina and Pseudomonas oleovorans isolated from oil contaminated soil in Jeddah city

  • Ebtihal A. Motwali Department of Biology, College of Science, Univirsity of Jeddah, Jeddah, Saudi Arabia.
  • Magda M. Aly Department of Biology, Faculity of Science, King Abdulaziz Univirsity, Jeddah, Saudi Arabia.
  • Huda A. Qari Department of Biology, Faculity of Science, King Abdulaziz Univirsity, Jeddah, Saudi Arabia.
  • Nuha M. Alhazmi Department of Biology, College of Science, Univirsity of Jeddah, Jeddah, Saudi Arabia.

Abstract

Objectives: The current study aimed to investigate the impact of different nutritional factors and growth conditions on biosurfactant production by bacterial isolates (EMB16 and EMB21) isolated from oil-contaminated soil samples.
Methods: Based on the used quantitative and qualitative screening method in current study, the selected bacterial isolates showed a high potential to produce biosurfactant. Results: By using 16S rRNA sequence analysis, the bacterial isolates EMB16 and EMB21 were found to be closely related to Pseudomonas mendocina and Pseudomonas oleovorans, respectively. The ultimate yields of biosurfactant (8.06±0.06 mg/ml) by Ps. mendocina EMB16 was with corn oil as a carbon source, urea as a nitrogen source, C/N ratio of 30, pH value of 7, and 2% inoculum size. For Ps. oleovorans EMB21, the maximum biosurfactant production (4.68±0.14mg/ml) was achieved by diesel oil as a carbon source, urea as a nitrogen source, C/N ratio of 30, pH of 7, and 5% size of inoculum. The best incubation period and temperature for the examined strains was 168 hrs. at 37℃.
Conclusion: The results proved that Ps. mendocina EMB16 was the most efficient biosurfactant producer as it showed the greatest amount of biosurfactant concentration and lowestvalue of surface tension measurement with an emulsification index of 67±6%.
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Published
2021-12-26
How to Cite
MOTWALI, Ebtihal A. et al. Effect of nutritional factors and growth conditions on biosurfactant production by Pseudomonas mendocina and Pseudomonas oleovorans isolated from oil contaminated soil in Jeddah city. Journal of Contemporary Medical Sciences, [S.l.], v. 7, n. 6, dec. 2021. ISSN 2413-0516. Available at: <http://www.jocms.org/index.php/jcms/article/view/1133>. Date accessed: 25 jan. 2022. doi: https://doi.org/10.22317/jcms.v7i6.1133.