Polymorphism of tumor necrosis factor-alpha 308 G/A gene in Iraqi patients with polycystic ovarian syndrome
AbstractObjective This study was designed to understand the etiological molecular role of tumor necrosis factor-alpha (TNF-a) 308 polymorphism as pro-inflammatory cytokine in the pathogenesis of this syndrome.Methods Genetic evaluation of 70 women with PCOS occur when the promoter region of the gene was amplified by polymerase chain reaction (PCR) and the presence or absence of the polymorphism at -308 was determined and compared with 30 healthy control women. In addition to the routine investigations and hormonal assay, this study includes the measurement of body mass index (BMI), lipid profileand glucose level in both patients and control group.Results It was found that TNF-a, 34.28% of (AA) allele, 2.85% of (AG) allele and 62.85% of (GG) allele this percentage detected in PCOS patients and compared with 20% of (AA) allele, 80% of (GG) allele with no (AG) alleles found in healthy control group (P > 0.05).Conclusion This study appeared no significant association between TNF-a gene polymorphism with PCOS. The relation of TNF-a 308 polymorphism with clinical and biochemical parameters examined and it found significantly linked with androgen excess in patients with PCOS as compared with control group. No correlation found for other clinical or biochemical measurements in PCOS and non PCOS groups.
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